Estradiol Activation of Uterine Reduced Diphosphopyridine Nucleotide Oxidase

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Estradiol activation of uterine reduced diphosphopyridine nucleotide oxidase.

Previous studies from this laboratory have shown that rat uterine homogenates contain a reduced pyridme nucleotide oxidase which is activated by ‘2,4-dichlorophenol and other phenolic substances (1, 2). Oophorectomy reduces the activity of this enzyme system to negligible levels but prompt restoration of the oxidative rate was observed upon the administration of small amounts of estradiol to th...

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Purification and properties of reduced diphosphopyridine nucleotide oxidase from Azotobacter.

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Enzymatic conversion of a reduced diphosphopyridine nucleotide derivative to reduced diphosphopyridine nucleotide.

DPNH-X was prepared as described previously (1) ; the barium salt was converted to the sodium salt before use, and solutions were stored at 20”. Concentrations of DPNH-X were calculated with an extinction coefficient of 13.5 X lo6 sq. cm. mole-’ at 290 rnM (1). Sodium salts of ADP, ATP, ITP, UTP, and crystalline disodium ATP were obtained from the Pabst Laboratories, Milwaukee, Wisconsin. Myoki...

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The need for additional study on the specific relation between metallic ions and metabolic function, especially in the case of the diphosphopyridine nucleotide oxidase system, has been expressed by Mahler (1) and others. This is particularly indicated in view of the fact that a number of the known components of this system contain iron. While it is broadly concluded that iron compounds facilita...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1960

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(18)69434-7